What does tmre mean

Log In. Know what is TMRE? Got another good explanation for TMRE? Don't keep it to yourself! Add it HERE! Still can't find the acronym definition you were looking for? Use our Power Search technology to look for more unique definitions from across the web!

Search the web. Citation Use the citation options below to add these abbreviations to your bibliography. Powered by CITE. Internet Directory Knowledge. I Don't Know. I Don't Kare. Incredible Dynamic Keyboard. Browse Abbreviations. Get instant explanation for any acronym or abbreviation that hits you anywhere on the web! Please let us know so that we can cite the reference in this datasheet.

What is your recommendation for plates to use with this assay? Thank you for contacting us. Please let me know if you have any other questions. Which one is more sensitive or more suitable for stem cells? Thank you for your inquiry. I heard back from the lab regardingthese two distinct dyes that both measure mitochondrial membrane potential. TMRE stains mitochondria only when there is a membrane potential. JC-1 is slightly different in that it has distinct emission spectra depending on whether mitochondria have high or low potential.

TMRE can be measured in a spectophotometer, fluorescent microscope or flow cytometry. JC-1 can be measured in a spectrophotometer. Details are in the respective protocol booklets. Flow cytometry is both sensitive and amenable to very few cells. I hope this information helps. Please contact us with any other questions.

Is it immediate, or is there a window after the incubation? The samples should be read immediately after the minute incubation with TMRE. Since the cells are live, as the health of the cell declined the signal from the dye would also diminish. Culture condition? Can we use this kit to measure mitochondrial membrane potential of yeast S. It all depends on the sample embryos vs. We have only worked with this dye using tissue culture cells monolayer or suspension.

If the samples are dissociated cells then TMRE labeling should work. The dye for measuring the potential in the kit is species independent, so it is more a matter of the sample type.

As for imaging tissue stained with TMRE, this is also tricky. Flow cytometry is almost certainly out due to the need for single cell suspension — which will be very difficult for most tissue types.

For microscopy I suppose it is possible with the proper microscope set up and the ability to adapt the live tissue to microscope stage constraints e. However, I would like to stress that this kit is only really for mitochondria labelling in intact cultured cells. Your name Your email. Send me a copy of this email. I agree to the terms and conditions. Cell Biology Apoptosis Mitochondrial.

TMRE is only suitable for use with live not fixed cells. Related assays Review the cell health assay guide to learn about kits to perform a cell viability assay , cytotoxicity assay and cell proliferation assay. Mitochondrial Membrane Potential is an important parameter of mitochondrial function used as an indicator of cell death.

The collapse of the mitochondrial Membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.

Associated products. FCCP, mitochondrial oxidative phosphorylation uncoupler ab